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KMID : 0380020030180040329
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Korean Journal of Biotechnology and Bioengineering
2003 Volume.18 No. 4 p.329 ~ p.334
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Regulatory Mechanism in Tissue-specific Expression of Insulin-liKe Growth Factor-1 Gene
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Ahn Mi-Ra
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Abstract
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The present study was aimed at investigating the regulatory mechanism in tissue-specific expression of insulin-like growth factor-I (IGF-I) gene. The expression of IGF-I gene was determined by a solution hybridization/RNase protection assay using total RNA prepared from rat liver or brain of various ages. The levels of IGF-I transcripts were increased in liver gradually after birth, but decreased in brain. By using an oligonucleotide (FRE) corresponding to the C/EBP binding site of the rat IGF-I exon 1, multiple forms of C/EBP and C/EBP proteins, which have DNA-binding activity, were detected in the rat liver or brain. Western immunoblot and southwestern analyses show that p42C/EBP/, p38C/EBP/, p35C/EBP/, p38C/EBP/, and p35C/EBP form specific complexes with the IGF-I exon 1 oligonucleotide in liver nuclear extract and that p42C/EBP/ and p38C/EBP/ form complexes in brain. These data suggest that the formation of FRE-C/EBP isoform complexes may play important roles in the tissue-specific regulation of IGF-I gene expression.
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KEYWORD
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Insulin-like growth factor-1, tissue-specific expression, C/EBPα, C/EBPβ
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